Genotyping and primer details


Genotyping and primer details for SALK_014639c - At2g43240
Line ID SALK_014639c
Locus At2g43240 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17971839 of line SALK_014639c Chr2:17971839
   
Insertion allele amplicon LBb3 - 13ty
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 13ty: 5'-CTCTCCTGGGTTCTGAATGTAAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17972389
   
wt amplicon (over InsPos) 28vb - 13ty
wt amplicon start Chr2:17971224
wt amplicon end Chr2:17972389
wt amplicon size 1166 bp
Locus specific primer 13ty: 5'-CTCTCCTGGGTTCTGAATGTAAAG-3'
2nd locus specific primer 28vb: 5'-TTCCAGATTATGATTGATGAGTGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11