Genotyping and primer details


Genotyping and primer details for SALK_016021c - At3g42950
Line ID SALK_016021c
Locus At3g42950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:15015795 of line SALK_016021c Chr3:15015795
   
Insertion allele amplicon LBb3 - 16gg
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 16gg: 5'-CTCTCACTCAATCGCTTTCTCTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:15015170
   
wt amplicon (over InsPos) 05hs - 16gg
wt amplicon start Chr3:15015170
wt amplicon end Chr3:15016145
wt amplicon size 976 bp
Locus specific primer 16gg: 5'-CTCTCACTCAATCGCTTTCTCTTT-3'
2nd locus specific primer 05hs: 5'-TTTCAGCTACTGCCTAAAAAGCTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11