Genotyping and primer details


Genotyping and primer details for SALK_017577c - At4g18890
Line ID SALK_017577c
Locus At4g18890 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:10352917 of line SALK_017577c Chr4:10352917
   
Insertion allele amplicon LBb3 - 03ca
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 03ca: 5'-GGTTGGAGATGCCTTTAAGACTAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:10351966
   
wt amplicon (over InsPos) 04ur - 03ca
wt amplicon start Chr4:10351966
wt amplicon end Chr4:10353482
wt amplicon size 1517 bp
Locus specific primer 03ca: 5'-GGTTGGAGATGCCTTTAAGACTAA-3'
2nd locus specific primer 04ur: 5'-CAAAGGAATCTAAACCCATTACCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11