Genotyping and primer details


Genotyping and primer details for SALK_018002c - At1g12380
Line ID SALK_018002c
Locus At1g12380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:4216501 of line SALK_018002c Chr1:4216501
   
Insertion allele amplicon LBb3 - 09vt
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 09vt: 5'-ATTCCTCAGTGCTTTGGTCTTAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:4215732
   
wt amplicon (over InsPos) 73wi - 09vt
wt amplicon start Chr1:4215732
wt amplicon end Chr1:4216966
wt amplicon size 1235 bp
Locus specific primer 09vt: 5'-ATTCCTCAGTGCTTTGGTCTTAAA-3'
2nd locus specific primer 73wi: 5'-TGTCACACGTAAAATCCAACTCTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11