Genotyping and primer details

Genotyping and primer details for SALK_018034 - At5g59470
Line ID SALK_018034
Locus At5g59470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:23979190 of line SALK_018034 Chr5:23979190
Insertion allele amplicon LBb3 - 52wx
Locus specific primer 52wx: 5'-CAACTTGAGGAGAAAACAATGGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:23978593
wt amplicon (over InsPos) 01xf - 52wx
wt amplicon start Chr5:23978593
wt amplicon end Chr5:23979677
wt amplicon size 1085 bp
Locus specific primer 52wx: 5'-CAACTTGAGGAGAAAACAATGGAT-3'
2nd locus specific primer 01xf: 5'-ATGACAAAGCCAAGGAGATTAGAA-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11