Genotyping and primer details


Genotyping and primer details for SALK_018512c - At3g02320
Line ID SALK_018512c
Locus At3g02320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:472985 of line SALK_018512c Chr3:472985
   
Insertion allele amplicon LBb3 - 27du
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 27du: 5'-GTCTTGGACATTATCTTCTGGCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:473438
   
wt amplicon (over InsPos) 09mu - 27du
wt amplicon start Chr3:472640
wt amplicon end Chr3:473438
wt amplicon size 799 bp
Locus specific primer 27du: 5'-GTCTTGGACATTATCTTCTGGCTC-3'
2nd locus specific primer 09mu: 5'-CTTACTACAGTCTCCGAGGCAAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11