Genotyping and primer details


Genotyping and primer details for SALK_018984c - At3g58700
Line ID SALK_018984c
Locus At3g58700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21712655 of line SALK_018984c Chr3:21712655
   
Insertion allele amplicon LBb3 - 03bt
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 03bt: 5'-GTCTGGTTTTCATATCAACCCTGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21711885
   
Insertion allele amplicon LBb3 - 06cl
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 06cl: 5'-TCTTCGATAACTTGGTAGGTGTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21711937
   
wt amplicon (over InsPos) 17cu - 06cl
wt amplicon start Chr3:21711937
wt amplicon end Chr3:21712744
wt amplicon size 808 bp
Locus specific primer 06cl: 5'-TCTTCGATAACTTGGTAGGTGTTG-3'
2nd locus specific primer 17cu: 5'-ATCTTTCGTCACTCTATGCTGGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11