Genotyping and primer details


Genotyping and primer details for SALK_019745c - At5g11630
Line ID SALK_019745c
Locus At5g11630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:3740557 of line SALK_019745c Chr5:3740557
   
Insertion allele amplicon LBb3 - 32vi
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 32vi: 5'-AATCTGTGCGGAGATTCTTTGTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:3740228
   
wt amplicon (over InsPos) 51vx - 32vi
wt amplicon start Chr5:3740228
wt amplicon end Chr5:3741347
wt amplicon size 1120 bp
Locus specific primer 32vi: 5'-AATCTGTGCGGAGATTCTTTGTAT-3'
2nd locus specific primer 51vx: 5'-CAATACAAGCATTCTTATGCCAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11