Genotyping and primer details


Genotyping and primer details for SALK_020762 - At2g42370
Line ID SALK_020762
Locus At2g42370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17644300 of line SALK_020762 Chr2:17644300
   
Insertion allele amplicon LBb3 - 35wm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 35wm: 5'-AAGCTTGTACCTTTCAAGCCATTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17643697
   
wt amplicon (over InsPos) 29xa - 35wm
wt amplicon start Chr2:17643697
wt amplicon end Chr2:17644849
wt amplicon size 1153 bp
Locus specific primer 35wm: 5'-AAGCTTGTACCTTTCAAGCCATTA-3'
2nd locus specific primer 29xa: 5'-ATCATTCTCGTGACCAAACTCTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11