Genotyping and primer details


Genotyping and primer details for SALK_021907 - At2g36220
Line ID SALK_021907
Locus At2g36220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:15193206 of line SALK_021907 Chr2:15193206
   
Insertion allele amplicon LBb3 - 46xl
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 46xl: 5'-TGTAGATGTAACAGTGGGAAGTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:15193977
   
Insertion allele amplicon R204 - CF78
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer CF78: 5'-TCCCTCTCTCTACACAAAGCTCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:15192531
   
wt amplicon (over InsPos) CF79 - CF78
wt amplicon start Chr2:15192531
wt amplicon end Chr2:15193254
wt amplicon size 724 bp
Locus specific primer CF78: 5'-TCCCTCTCTCTACACAAAGCTCTC-3'
2nd locus specific primer CF79: 5'-GAATACCGACTTCTCATCAGCTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11