Genotyping and primer details


Genotyping and primer details for SALK_022152 - At3g09680
Line ID SALK_022152
Locus At3g09680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2969951 of line SALK_022152 Chr3:2969951
   
Insertion allele amplicon LBb3 - 49us
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 49us: 5'-CCACTGCATAATCAAACACAGAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2969544
   
wt amplicon (over InsPos) 27vx - 49us
wt amplicon start Chr3:2969544
wt amplicon end Chr3:2970552
wt amplicon size 1009 bp
Locus specific primer 49us: 5'-CCACTGCATAATCAAACACAGAGT-3'
2nd locus specific primer 27vx: 5'-TAAGGAGTTGGCATAATATTTGGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11