Genotyping and primer details


Genotyping and primer details for SALK_022835c - At3g10860
Line ID SALK_022835c
Locus At3g10860 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3400247 of line SALK_022835c Chr3:3400247
   
Insertion allele amplicon LBb3 - 11rb
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 11rb: 5'-CTGATGATGAACCGAGTCAGATTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3399412
   
wt amplicon (over InsPos) 22rz - 11rb
wt amplicon start Chr3:3399412
wt amplicon end Chr3:3400393
wt amplicon size 982 bp
Locus specific primer 11rb: 5'-CTGATGATGAACCGAGTCAGATTA-3'
2nd locus specific primer 22rz: 5'-CCGTAATGTTTAGATCGATCAGGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11