Genotyping and primer details


Genotyping and primer details for SALK_024490c - At1g68440
Line ID SALK_024490c
Locus At1g68440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:25658647 of line SALK_024490c Chr1:25658647
   
Insertion allele amplicon LBb3 - 75by
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 75by: 5'-AAATTATCATCAATGATCCGCTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:25657634
   
wt amplicon (over InsPos) 02dd - 75by
wt amplicon start Chr1:25657634
wt amplicon end Chr1:25658680
wt amplicon size 1047 bp
Locus specific primer 75by: 5'-AAATTATCATCAATGATCCGCTTC-3'
2nd locus specific primer 02dd: 5'-TCGATATCTAAATGATCCCAAAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11