Genotyping and primer details


Genotyping and primer details for SALK_024656 - At5g51590
Line ID SALK_024656
Locus At5g51590 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:20958427 of line SALK_024656 Chr5:20958427
   
Insertion allele amplicon LBb3 - 12rb
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 12rb: 5'-AATTATCGAAAACATACCTCACCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:20957895
   
wt amplicon (over InsPos) 21rz - 12rb
wt amplicon start Chr5:20957895
wt amplicon end Chr5:20958964
wt amplicon size 1070 bp
Locus specific primer 12rb: 5'-AATTATCGAAAACATACCTCACCG-3'
2nd locus specific primer 21rz: 5'-CTCTTCCTTAATTGTCGGAATCAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11