Genotyping and primer details


Genotyping and primer details for SALK_025428c - At5g27760
Line ID SALK_025428c
Locus At5g27760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:9830660 of line SALK_025428c Chr5:9830660
   
Insertion allele amplicon LBb3 - 34vi
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 34vi: 5'-TTAAATTTTGATGGCTTTGGAATC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:9830166
   
wt amplicon (over InsPos) 53vw - 34vi
wt amplicon start Chr5:9830166
wt amplicon end Chr5:9831381
wt amplicon size 1216 bp
Locus specific primer 34vi: 5'-TTAAATTTTGATGGCTTTGGAATC-3'
2nd locus specific primer 53vw: 5'-CCAAACATGGTAAAGAACCAATCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11