Genotyping and primer details


Genotyping and primer details for SALK_025557c - At3g03480
Line ID SALK_025557c
Locus At3g03480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:828717 of line SALK_025557c Chr3:828717
   
Insertion allele amplicon LBb3 - 46fm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 46fm: 5'-TTTCTGAAATCACTATGCGAGTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:829212
   
wt amplicon (over InsPos) 10gs - 46fm
wt amplicon start Chr3:828201
wt amplicon end Chr3:829212
wt amplicon size 1012 bp
Locus specific primer 46fm: 5'-TTTCTGAAATCACTATGCGAGTTG-3'
2nd locus specific primer 10gs: 5'-CCTCCGTCTACCAAAACATAAAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11