Genotyping and primer details


Genotyping and primer details for SALK_026580c - At2g22230
Line ID SALK_026580c
Locus At2g22230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:9450664 of line SALK_026580c Chr2:9450664
   
Insertion allele amplicon LBb3 - 44sf
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 44sf: 5'-ACCTCTACCAAATTAGCGAGATCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:9451051
   
wt amplicon (over InsPos) 16tl - 44sf
wt amplicon start Chr2:9450255
wt amplicon end Chr2:9451051
wt amplicon size 797 bp
Locus specific primer 44sf: 5'-ACCTCTACCAAATTAGCGAGATCA-3'
2nd locus specific primer 16tl: 5'-CTCTCGATTTCTAATGTTTCCGAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11