Genotyping and primer details


Genotyping and primer details for SALK_026969c - At5g47730
Line ID SALK_026969c
Locus At5g47730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:19336766 of line SALK_026969c Chr5:19336766
   
Insertion allele amplicon LBb3 - 19ea
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 19ea: 5'-TGAAATCCCAAACTAATAACACCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:19337274
   
wt amplicon (over InsPos) 51gc - 19ea
wt amplicon start Chr5:19336450
wt amplicon end Chr5:19337274
wt amplicon size 825 bp
Locus specific primer 19ea: 5'-TGAAATCCCAAACTAATAACACCC-3'
2nd locus specific primer 51gc: 5'-CATAAGTTTTCTTCAGTGGCTCCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11