Genotyping and primer details


Genotyping and primer details for SALK_028578c - At4g01950
Line ID SALK_028578c
Locus At4g01950 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:845693 of line SALK_028578c Chr4:845693
   
Insertion allele amplicon LBb3 - 13jg
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 13jg: 5'-TTTTGCCAGGTCAGTCTCTTTTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:846038
   
wt amplicon (over InsPos) 08km - 13jg
wt amplicon start Chr4:845354
wt amplicon end Chr4:846038
wt amplicon size 685 bp
Locus specific primer 13jg: 5'-TTTTGCCAGGTCAGTCTCTTTTAT-3'
2nd locus specific primer 08km: 5'-AAAGTGTTCATTAGGGTTGGTTTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11