Genotyping and primer details


Genotyping and primer details for SALK_032288c - At3g24360
Line ID SALK_032288c
Locus At3g24360 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:8841858 of line SALK_032288c Chr3:8841858
   
Insertion allele amplicon LBb3 - 64kh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 64kh: 5'-TTGGCTGTATCAAGGCTTATCTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:8842151
   
wt amplicon (over InsPos) 04lz - 64kh
wt amplicon start Chr3:8841364
wt amplicon end Chr3:8842151
wt amplicon size 788 bp
Locus specific primer 64kh: 5'-TTGGCTGTATCAAGGCTTATCTCT-3'
2nd locus specific primer 04lz: 5'-CCACACAACAGTACATAACAAGGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11