Genotyping and primer details


Genotyping and primer details for SALK_032652c - At4g39610
Line ID SALK_032652c
Locus At4g39610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:18394010 of line SALK_032652c Chr4:18394010
   
Insertion allele amplicon LBb3 - 33dr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 33dr: 5'-TTCATGAATTCACATCTTTTCTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:18393220
   
wt amplicon (over InsPos) 04ev - 33dr
wt amplicon start Chr4:18393220
wt amplicon end Chr4:18394118
wt amplicon size 899 bp
Locus specific primer 33dr: 5'-TTCATGAATTCACATCTTTTCTGC-3'
2nd locus specific primer 04ev: 5'-CCGAGAAGAAGACAGATATTTTGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11