Genotyping and primer details


Genotyping and primer details for SALK_035555c - At5g04710
Line ID SALK_035555c
Locus At5g04710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1359363 of line SALK_035555c Chr5:1359363
   
Insertion allele amplicon LBb3 - 02ca
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 02ca: 5'-AAAACGAAGACTGGAATCTGAAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1359748
   
Insertion allele amplicon LBb3 - CE68
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer CE68: 5'-TTCATCTGATTTTGTTGCCAGTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1359015
   
wt amplicon (over InsPos) CE68 - 02ca
wt amplicon start Chr5:1359015
wt amplicon end Chr5:1359748
wt amplicon size 734 bp
Locus specific primer 02ca: 5'-AAAACGAAGACTGGAATCTGAAAC-3'
2nd locus specific primer CE68: 5'-TTCATCTGATTTTGTTGCCAGTAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11