Genotyping and primer details


Genotyping and primer details for SALK_036792 - At4g27680
Line ID SALK_036792
Locus At4g27680 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13823081 of line SALK_036792 Chr4:13823081
   
Insertion allele amplicon LBb3 - 13ke
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 13ke: 5'-TTCAGTGTTGCATCCACTAGTTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13822782
   
wt amplicon (over InsPos) 10ll - 13ke
wt amplicon start Chr4:13822782
wt amplicon end Chr4:13823606
wt amplicon size 825 bp
Locus specific primer 13ke: 5'-TTCAGTGTTGCATCCACTAGTTTC-3'
2nd locus specific primer 10ll: 5'-GAAAAGTAACAAACGAAATTGGGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11