Genotyping and primer details


Genotyping and primer details for SALK_037136 - At4g29920
Line ID SALK_037136
Locus At4g29920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14632699 of line SALK_037136 Chr4:14632699
   
Insertion allele amplicon LBb3 - 54us
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 54us: 5'-TAATGGAATGAAAAGGTGACAACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14632406
   
wt amplicon (over InsPos) 29vl - 54us
wt amplicon start Chr4:14632406
wt amplicon end Chr4:14633297
wt amplicon size 892 bp
Locus specific primer 54us: 5'-TAATGGAATGAAAAGGTGACAACA-3'
2nd locus specific primer 29vl: 5'-GGTTTGGTCAATAACAAGAGGAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11