Genotyping and primer details


Genotyping and primer details for SALK_037762 - At1g21440
Line ID SALK_037762
Locus At1g21440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7503164 of line SALK_037762 Chr1:7503164
   
Insertion allele amplicon LBb3 - AD02
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer AD02: 5'-CGATTGTGGACAGAGTAATCAGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7502296
   
wt amplicon (over InsPos) 03lt - AD02
wt amplicon start Chr1:7502296
wt amplicon end Chr1:7503853
wt amplicon size 1558 bp
Locus specific primer AD02: 5'-CGATTGTGGACAGAGTAATCAGAA-3'
2nd locus specific primer 03lt: 5'-CCGTAACTTTGGGAAAACCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11