Genotyping and primer details


Genotyping and primer details for SALK_039631c - At5g59750
Line ID SALK_039631c
Locus At5g59750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:24073560 of line SALK_039631c Chr5:24073560
   
Insertion allele amplicon R204 - 52aq
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer 52aq: 5'-ATTTTGCATCCTGTAACCAAGAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24074145
   
wt amplicon (over InsPos) 24bi - 52aq
wt amplicon start Chr5:24073146
wt amplicon end Chr5:24074145
wt amplicon size 1000 bp
Locus specific primer 52aq: 5'-ATTTTGCATCCTGTAACCAAGAAC-3'
2nd locus specific primer 24bi: 5'-GGGTTTCCGTTTATGTGATAGTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11