Genotyping and primer details


Genotyping and primer details for SALK_040274c - At4g05000
Line ID SALK_040274c
Locus At4g05000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:2564437 of line SALK_040274c Chr4:2564437
   
Insertion allele amplicon LBb3 - 17td
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 17td: 5'-AGGTCAAATTATGGAACGACAAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:2563960
   
wt amplicon (over InsPos) 10ua - 17td
wt amplicon start Chr4:2563960
wt amplicon end Chr4:2564701
wt amplicon size 742 bp
Locus specific primer 17td: 5'-AGGTCAAATTATGGAACGACAAAC-3'
2nd locus specific primer 10ua: 5'-CATAAGCACATTCAACTCCTCAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11