Genotyping and primer details


Genotyping and primer details for SALK_040526c - At2g35765
Line ID SALK_040526c
Locus At2g35765 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:15033475 of line SALK_040526c Chr2:15033475
   
Insertion allele amplicon LBb3 - 18td
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 18td: 5'-CCTCTTCGCTCATAAACAAATCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:15033978
   
wt amplicon (over InsPos) 12ue - 18td
wt amplicon start Chr2:15033116
wt amplicon end Chr2:15033978
wt amplicon size 863 bp
Locus specific primer 18td: 5'-CCTCTTCGCTCATAAACAAATCTC-3'
2nd locus specific primer 12ue: 5'-CAACCTTGTTTGTGGAACCTCTAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11