Genotyping and primer details


Genotyping and primer details for SALK_040744c - At5g61570
Line ID SALK_040744c
Locus At5g61570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:24758641 of line SALK_040744c Chr5:24758641
   
Insertion allele amplicon R204 - 04as
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer 04as: 5'-TAAAACCCTAACAGAGGAACGAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:24758949
   
wt amplicon (over InsPos) 03bp - 04as
wt amplicon start Chr5:24758193
wt amplicon end Chr5:24758949
wt amplicon size 757 bp
Locus specific primer 04as: 5'-TAAAACCCTAACAGAGGAACGAGA-3'
2nd locus specific primer 03bp: 5'-CAGGGGAATAAAAACACACAAGTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11