Genotyping and primer details


Genotyping and primer details for SALK_041461c - At2g28725
Line ID SALK_041461c
Locus At2g28725 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:12328888 of line SALK_041461c Chr2:12328888
   
Insertion allele amplicon LBb3 - 11tw
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 11tw: 5'-TGTAAGAGTCCTCTCTCCCACTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:12328488
   
wt amplicon (over InsPos) 11ue - 11tw
wt amplicon start Chr2:12328488
wt amplicon end Chr2:12329352
wt amplicon size 865 bp
Locus specific primer 11tw: 5'-TGTAAGAGTCCTCTCTCCCACTCT-3'
2nd locus specific primer 11ue: 5'-AATCGAATGACAAATATGGACTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11