Genotyping and primer details


Genotyping and primer details for SALK_041720 - At3g61580
Line ID SALK_041720
Locus At3g61580 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22786621 of line SALK_041720 Chr3:22786621
   
Insertion allele amplicon LBb3 - 55bk
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 55bk: 5'-TAAGGTAACACGTGGTCTGTCTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22785754
   
wt amplicon (over InsPos) 15cr - 55bk
wt amplicon start Chr3:22785754
wt amplicon end Chr3:22786751
wt amplicon size 998 bp
Locus specific primer 55bk: 5'-TAAGGTAACACGTGGTCTGTCTTG-3'
2nd locus specific primer 15cr: 5'-TAACGTAATGACCAGAATCGTGAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11