Genotyping and primer details


Genotyping and primer details for SALK_042866c - At1g06660
Line ID SALK_042866c
Locus At1g06660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:2039760 of line SALK_042866c Chr1:2039760
   
Insertion allele amplicon LBb3 - 18ty
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 18ty: 5'-GAAAAATTTCAGAGCTTCCACAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:2039049
   
wt amplicon (over InsPos) 26uu - 18ty
wt amplicon start Chr1:2039049
wt amplicon end Chr1:2040140
wt amplicon size 1092 bp
Locus specific primer 18ty: 5'-GAAAAATTTCAGAGCTTCCACAAC-3'
2nd locus specific primer 26uu: 5'-ACTGTTGATGAACCGAAACCTAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11