Genotyping and primer details


Genotyping and primer details for SALK_045266 - At3g25585
Line ID SALK_045266
Locus At3g25585 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:9298101 of line SALK_045266 Chr3:9298101
   
Insertion allele amplicon LBb3 - 78by
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 78by: 5'-CATTTCCAGCAGTGAGAATCATAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:9298707
   
wt amplicon (over InsPos) 13dk - 78by
wt amplicon start Chr3:9297507
wt amplicon end Chr3:9298707
wt amplicon size 1201 bp
Locus specific primer 78by: 5'-CATTTCCAGCAGTGAGAATCATAA-3'
2nd locus specific primer 13dk: 5'-GCAGGAGTTCTGATTTGGTAAGTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11