Genotyping and primer details


Genotyping and primer details for SALK_047689c - At4g25980
Line ID SALK_047689c
Locus At4g25980 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13190720 of line SALK_047689c Chr4:13190720
   
Insertion allele amplicon LBb3 - 06tw
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 06tw: 5'-CAGGTAAGAAATCATAGAGCCGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13191151
   
wt amplicon (over InsPos) 10ue - 06tw
wt amplicon start Chr4:13190309
wt amplicon end Chr4:13191151
wt amplicon size 843 bp
Locus specific primer 06tw: 5'-CAGGTAAGAAATCATAGAGCCGTT-3'
2nd locus specific primer 10ue: 5'-AAAGTTTTTGGTTCGAAGTTCAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11