Genotyping and primer details


Genotyping and primer details for SALK_047972c - At2g32280
Line ID SALK_047972c
Locus At2g32280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:13713831 of line SALK_047972c Chr2:13713831
   
Insertion allele amplicon LBb3 - 16og
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 16og: 5'-TTAATTTAAAGACCCGAAGCAAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:13714243
   
wt amplicon (over InsPos) 01om - 16og
wt amplicon start Chr2:13713297
wt amplicon end Chr2:13714243
wt amplicon size 947 bp
Locus specific primer 16og: 5'-TTAATTTAAAGACCCGAAGCAAAG-3'
2nd locus specific primer 01om: 5'-GCCCAAAATCAGGTAAAATAAATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11