Genotyping and primer details


Genotyping and primer details for SALK_049540c - At3g50410
Line ID SALK_049540c
Locus At3g50410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18710109 of line SALK_049540c Chr3:18710109
   
Insertion allele amplicon LBb3 - 81sm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 81sm: 5'-CTTCATAGGGATGAAAAACAGAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18709515
   
wt amplicon (over InsPos) 15tl - 81sm
wt amplicon start Chr3:18709515
wt amplicon end Chr3:18710638
wt amplicon size 1124 bp
Locus specific primer 81sm: 5'-CTTCATAGGGATGAAAAACAGAGC-3'
2nd locus specific primer 15tl: 5'-TTTTCTCATTTGAGTGAGTTTCCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11