Genotyping and primer details


Genotyping and primer details for SALK_050243c - At4g00390
Line ID SALK_050243c
Locus At4g00390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:171511 of line SALK_050243c Chr4:171511
   
Insertion allele amplicon LBb3 - 29ux
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 29ux: 5'-TTCAAAGACTATGGAGTGACGATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:172275
   
wt amplicon (over InsPos) 43fb - 29ux
wt amplicon start Chr4:171320
wt amplicon end Chr4:172275
wt amplicon size 955 bp
Locus specific primer 29ux: 5'-TTCAAAGACTATGGAGTGACGATG-3'
2nd locus specific primer 43fb: 5'-CATTTTCAAACTCCAACTCTCACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11