Genotyping and primer details


Genotyping and primer details for SALK_050877c - At2g45100
Line ID SALK_050877c
Locus At2g45100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18595319 of line SALK_050877c Chr2:18595319
   
Insertion allele amplicon LBb3 - 02ha
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 02ha: 5'-GTTTCTTTTGTAGAAGGAAGCAGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18595900
   
wt amplicon (over InsPos) 04ho - 02ha
wt amplicon start Chr2:18595163
wt amplicon end Chr2:18595900
wt amplicon size 738 bp
Locus specific primer 02ha: 5'-GTTTCTTTTGTAGAAGGAAGCAGC-3'
2nd locus specific primer 04ho: 5'-AATCATTTTTCCCACAAAAACAGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11