Genotyping and primer details


Genotyping and primer details for SALK_054337c - At1g53730
Line ID SALK_054337c
Locus At1g53730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:20064513 of line SALK_054337c Chr1:20064513
   
Insertion allele amplicon R204 - BL03
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer BL03: 5'-TCTCCCAGTTAAAAGTTCAAGCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20064988
   
Insertion allele amplicon LBb3 - BL04
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer BL04: 5'-ACTGGCAGTTTCAGCGTAGATAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20064131
   
wt amplicon (over InsPos) BL04 - BL03
wt amplicon start Chr1:20064131
wt amplicon end Chr1:20064988
wt amplicon size 858 bp
Locus specific primer BL03: 5'-TCTCCCAGTTAAAAGTTCAAGCAT-3'
2nd locus specific primer BL04: 5'-ACTGGCAGTTTCAGCGTAGATAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11