Genotyping and primer details


Genotyping and primer details for SALK_054396 - At1g73570
Line ID SALK_054396
Locus At1g73570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27653828 of line SALK_054396 Chr1:27653828
   
Insertion allele amplicon LBb3 - 14jg
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 14jg: 5'-TATGCTAAATCTCAATCAAATGCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27653432
   
wt amplicon (over InsPos) 03kp - 14jg
wt amplicon start Chr1:27653432
wt amplicon end Chr1:27654414
wt amplicon size 983 bp
Locus specific primer 14jg: 5'-TATGCTAAATCTCAATCAAATGCG-3'
2nd locus specific primer 03kp: 5'-ATCAGCTTTCATTCGGGTAAAATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11