Genotyping and primer details


Genotyping and primer details for SALK_054530c - At1g07180
Line ID SALK_054530c
Locus At1g07180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:2205963 of line SALK_054530c Chr1:2205963
   
Insertion allele amplicon LBb3 - 81by
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 81by: 5'-ACGAGAGCTTTGTATCTCCCAATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:2206499
   
wt amplicon (over InsPos) 12da - 81by
wt amplicon start Chr1:2205489
wt amplicon end Chr1:2206499
wt amplicon size 1011 bp
Locus specific primer 81by: 5'-ACGAGAGCTTTGTATCTCCCAATA-3'
2nd locus specific primer 12da: 5'-GTGAAGATGAGAAGAAGAGGCTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11