Genotyping and primer details


Genotyping and primer details for SALK_055283c - At4g25970
Line ID SALK_055283c
Locus At4g25970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13185241 of line SALK_055283c Chr4:13185241
   
Insertion allele amplicon LBb3 - 03tw
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 03tw: 5'-AGATGATTTTCCTTTTCTTGATGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13184927
   
wt amplicon (over InsPos) 09ue - 03tw
wt amplicon start Chr4:13184927
wt amplicon end Chr4:13185553
wt amplicon size 627 bp
Locus specific primer 03tw: 5'-AGATGATTTTCCTTTTCTTGATGC-3'
2nd locus specific primer 09ue: 5'-AGGTTAAAATCACAAAGCATCACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11