Genotyping and primer details


Genotyping and primer details for SALK_057452 - At4g17486
Line ID SALK_057452
Locus At4g17486 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:9751273 of line SALK_057452 Chr4:9751273
   
Insertion allele amplicon LBb3 - 14ck
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 14ck: 5'-ACTTTTCTCGATCATTTTTGCAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:9751692
   
wt amplicon (over InsPos) 06dq - 14ck
wt amplicon start Chr4:9750840
wt amplicon end Chr4:9751692
wt amplicon size 853 bp
Locus specific primer 14ck: 5'-ACTTTTCTCGATCATTTTTGCAGT-3'
2nd locus specific primer 06dq: 5'-CCCCTTCTCAAAAGAGCCTAGTAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11