Genotyping and primer details


Genotyping and primer details for SALK_057856c - At5g19310
Line ID SALK_057856c
Locus At5g19310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:6498808 of line SALK_057856c Chr5:6498808
   
Insertion allele amplicon LBb3 - 48fm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 48fm: 5'-TTTCCTGCAAATCTTCACCTCTAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:6499562
   
wt amplicon (over InsPos) 02hf - 48fm
wt amplicon start Chr5:6498743
wt amplicon end Chr5:6499562
wt amplicon size 820 bp
Locus specific primer 48fm: 5'-TTTCCTGCAAATCTTCACCTCTAT-3'
2nd locus specific primer 02hf: 5'-AGCGACTAAATAATCGTTCGTGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11