Genotyping and primer details


Genotyping and primer details for SALK_058044c - At3g13810
Line ID SALK_058044c
Locus At3g13810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:4546942 of line SALK_058044c Chr3:4546942
   
Insertion allele amplicon LBb3 - 31de
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 31de: 5'-ATTCATCAATCTGCTTCTCATCCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:4546509
   
wt amplicon (over InsPos) 06dz - 31de
wt amplicon start Chr3:4546509
wt amplicon end Chr3:4547243
wt amplicon size 735 bp
Locus specific primer 31de: 5'-ATTCATCAATCTGCTTCTCATCCT-3'
2nd locus specific primer 06dz: 5'-TCCAAGACCAGCAAAACTTAGAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11