Genotyping and primer details


Genotyping and primer details for SALK_058566 - At1g55310
Line ID SALK_058566
Locus At1g55310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:20632428 of line SALK_058566 Chr1:20632428
   
Insertion allele amplicon LBb3 - 75hw
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 75hw: 5'-AAACATAAAGTTCTCGGCTTTTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:20632802
   
wt amplicon (over InsPos) 03jt - 75hw
wt amplicon start Chr1:20631992
wt amplicon end Chr1:20632802
wt amplicon size 811 bp
Locus specific primer 75hw: 5'-AAACATAAAGTTCTCGGCTTTTCA-3'
2nd locus specific primer 03jt: 5'-TGTTTGCAGAAGAGAACAGAAAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11