Genotyping and primer details


Genotyping and primer details for SALK_059416c - At1g27400
Line ID SALK_059416c
Locus At1g27400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:9515350 of line SALK_059416c Chr1:9515350
   
Insertion allele amplicon LBb3 - 16vo
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 16vo: 5'-TGACAATACATTCACGAAATCCAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:9514679
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11