Genotyping and primer details


Genotyping and primer details for SALK_059944c - At1g75060
Line ID SALK_059944c
Locus At1g75060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28182396 of line SALK_059944c Chr1:28182396
   
Insertion allele amplicon LBb3 - 39um
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 39um: 5'-CACCAAGTAGAAGAAATTTGAGGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28181944
   
wt amplicon (over InsPos) 31vb - 39um
wt amplicon start Chr1:28181944
wt amplicon end Chr1:28182766
wt amplicon size 823 bp
Locus specific primer 39um: 5'-CACCAAGTAGAAGAAATTTGAGGC-3'
2nd locus specific primer 31vb: 5'-GAATCCTTATCTTTGTGGGTTCAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11