Genotyping and primer details


Genotyping and primer details for SALK_060037c - At1g72210
Line ID SALK_060037c
Locus At1g72210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27181553 of line SALK_060037c Chr1:27181553
   
Insertion allele amplicon LBb3 - 40um
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 40um: 5'-TTCGAGGAAGAAGCTTATGAATGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27182069
   
wt amplicon (over InsPos) 30vb - 40um
wt amplicon start Chr1:27181039
wt amplicon end Chr1:27182069
wt amplicon size 1031 bp
Locus specific primer 40um: 5'-TTCGAGGAAGAAGCTTATGAATGT-3'
2nd locus specific primer 30vb: 5'-AGACTAACATAAGGTTGGTGGTGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11