Genotyping and primer details


Genotyping and primer details for SALK_060839 - At1g17450
Line ID SALK_060839
Locus At1g17450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5992291 of line SALK_060839 Chr1:5992291
   
Insertion allele amplicon LBb3 - 33ux
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 33ux: 5'-TGCCAAATAATTTTCTTTTTGTCC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5992826
   
wt amplicon (over InsPos) 21wq - 33ux
wt amplicon start Chr1:5991575
wt amplicon end Chr1:5992826
wt amplicon size 1252 bp
Locus specific primer 33ux: 5'-TGCCAAATAATTTTCTTTTTGTCC-3'
2nd locus specific primer 21wq: 5'-CATTGAAGTCATCCCATTTTTCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11